Journal: Biology of reproduction

Article Title: Distribution of immune cells in the epididymis of the aging Brown Norway rat is segment-specific and related to the luminal content.

doi: 10.1095/biolreprod61.3.705

Figure Lengend Snippet: FIG. 1. Light micrographs showing sec- tions of the epididymis of the Brown Nor- way rat stained with an antibody for monocytes-macrophages (ED1). A–C) Cor- pus, D–F) distal cauda. A, D) 3 mo, B, E) 18 mo with numerous spermatozoa in the lumen, C, F) 18 mo with occasional sper- matozoa in the lumen. lu, Lumen; it, inter- tubular space; p, principal cells; b, basal cells; c, clear cells; bm, basement mem- brane. Arrows, ED1-positive cells; aster- isks, halo cells. Scale bar, A–F 5 64 mm.

Article Snippet: Mouse anti-rat ED1 (Serotec USA, Washington, DC) was used at a dilution 1/100; this antibody recognizes a cytoplasmic antigen in monocytes and most macrophages.

Techniques: Staining

Journal: Biology of reproduction

Article Title: Distribution of immune cells in the epididymis of the aging Brown Norway rat is segment-specific and related to the luminal content.

doi: 10.1095/biolreprod61.3.705

Figure Lengend Snippet: FIG. 5. Light micrographs showing sec- tions of the corpus of the epididymis of Brown Norway rats. A, C) Three mo; B, D) 18 mo. Section stained with an antibody for A and B GST Yf and C and D ED1. Clear arrows, basal cells; dark arrows, ED1-positive cells; other labels as in Fig- ure 1. Scale bar, A–D 5 64 mm.

Article Snippet: Mouse anti-rat ED1 (Serotec USA, Washington, DC) was used at a dilution 1/100; this antibody recognizes a cytoplasmic antigen in monocytes and most macrophages.

Techniques: Staining

Journal: iScience

Article Title: TMEM106B reduction does not rescue GRN deficiency in iPSC-derived human microglia and mouse models

doi: 10.1016/j.isci.2023.108362

Figure Lengend Snippet:

Article Snippet: IHC staining was performed using rat anti-Lamp1 (Abcam #ab25245), goat anti-ChAT (choline acetyltransferase; Millipore #AB144P, AB_2079751), rabbit anti-CatD (Abcam #AB75852), rabbit anti-dMBP (Millipore #AB5864, AB_2140351), rabbit anti-Iba1 (Wako #019–19741, AB_839504), rat anti-CD68 (Bio-Rad #MCA1957T, AB_2074849) counterstained with Nissl (0.05% thionine/0.08M acetate buffer, pH 4.5, Amino cupric silver (de Olmos Stain for degenerating cells), or with solochrome stain (for myelin).

Techniques: Staining, Recombinant, Protease Inhibitor, Sequencing, Modification, Bradford Protein Assay, Software, Mass Spectrometry

Journal: Cell Death & Disease

Article Title: Large triglyceride-rich lipoproteins in hypertriglyceridemia are associated with the severity of acute pancreatitis in experimental mice

doi: 10.1038/s41419-019-1969-3

Figure Lengend Snippet: a Triglyceride concentration of plasma before caerulein treatment between wild-type, HTG1 (triglyceride concentration, 1000–2000 mg/dL) and HTG2 mice(triglyceride concentration, å 2000 mg/dL). b , c Plasma amylase and lipase activity at 12 th hour after the first injection of caerulein (50 μg/kg, 10 times, hourly) between these three groups. d Representative photomicrographs of H&E-stained section of pancreas in wild-type, HTG1 and HTG2 mice after acute pancreatitis induction. Scale bar = 500 or 100 μm. e Pathological scores of the pancreas in wild-type, HTG1 and HTG2 mice after acute pancreatitis induction. f Incidence rates of pancreatic necrosis between these three groups after acute pancreatitis induction. Each value was the mean ± SEM for n = 6. g Immunohistochemistry evaluation for myeloperoxidase and CD68 in pancreas between wild-type, HTG1 and HTG2 mice after acute pancreatitis induction. Scale bar = 100 μm. i Semiquantitative results of the area ratio of myeloperoxidase and CD68-positive cells among wild-type, HTG1 and HTG2 mice after acute pancreatitis induction. Each value was the mean ± SEM for n = 3–5. * p < 0.05 or ** p < 0.01 or *** p < 0.001 vs wild-type group. # p < 0.05 or ## p < 0.01 or ### p < 0.001 vs HTG1 group. WT wild-type, TG triglyceride, Ed edema, Necr necrosis, Vac vacuolisation, Infl inflammation, FFAs free fatty acids, MPO myeloperoxidase

Article Snippet: Neutrophil and macrophage infiltration were evaluated by immunohistochemical staining using anti-myeloperoxidase rabbit polyclonal antibody (Abcam, ab9535) and anti-CD68 rabbit polyclonal antibody (Boster, BA3638), respectively.

Techniques: Concentration Assay, Clinical Proteomics, Activity Assay, Injection, Staining, Immunohistochemistry